148 Dokumente gefunden
Polar microcystins or arginine methyl ester can serve as sensitive reference materials for system suitability tests in untargeted…
aus: Metabolomics
Introduction: LC-MS system suitability test (SST) is crucial for reliable data acquisition especially in untargeted metabolomics. Objectives: Identification of best reference materials (RMs) to improve best quality assurance (QA) and quality control (QC) practices. Methods: Investigations were performed…
Berlin Heidelberg: Springer US, 2026-03-07
Epigenetic Regulation Links Bacterial Signaling to Growth and Reproductive Development in the Green Macroalga Ulva compressa…
aus: Marine Biotechnology
DNA (deoxyribonucleic acid ) methylation is a key epigenetic mechanism that regulates gene expression and developmental transitions in eukaryotes. Interestingly, algal growth- and morphogenesis-promoting bacteria can reduce global DNA methylation levels in the green seaweed Ulva (Chlorophyta), highlighting…
New York: Springer, 2026-03-04
Comparability of Liquid Chromatography Tandem Mass Spectrometry Analysis of Dissolved Organic Matter across Laboratories
Non-targeted liquid chromatography tandem high-resolution mass spectrometry (LC–MS/MS) is increasingly applied for the structure-resolved chemical analysis of dissolved organic matter (DOM). With new developments in MS instrumentation and analysis software, the approach has gained substantial momentum…
Columbus: American Chemical Society, 2026-02-06
Global DNA-methylation in quantitative epigenetics : orbitrap mass spectrometry
DNA methylation is the most common epigenetic modification in both prokaryotic and eukaryotic genomes. Here we present a method based on highly efficient acid-hydrolysis of DNA, liquid chromatography, and detection by mass spectrometry to accurately quantify cytosine methylation in highly methylated…
Lausanne: Frontiers, 2025-09-26
Groundwater microbiomes balance resilience and vulnerability to hydroclimatic extremes
Groundwater health is increasingly threatened by climate change, which alters precipitation patterns, leading to groundwater recharge shifts. These shifts impact subsurface microbial communities, crucial for maintaining ecosystem functions. In this decade-long study of carbonate aquifers, we analyzed…
London: Nature Publishing Group UK, 2025-08-20
Elucidation and engineering of two distinct plant terpene alkaloid biosynthetic pathways
Numerous monoterpene indole alkaloids (MIAs) and steroidal glycoalkaloids (SGAs) are highly valuable due to their beneficial bioactivities. For example, vinblastine is an MIA produced by the plant Catharanthus roseus that is used as an anti-cancer agent, serpentine is a topoisomerase inhibitor also produced…
Recent Advances in Understanding the Sex Pheromone‐Mediated Communication of Diatoms
aus: ChemPlusChem
Diatoms are prolific unicellular microalgae, contributing ≈20% of global photosynthetic CO 2 fixation. These algae form the base of the marine food web. They are also widely distributed in freshwater and utilized in aquaculture. Despite their ecological and economic significance, many fundamental aspects…
Weinheim: Wiley-VCH, 2025-08-14
Non-cyanobacterial diazotrophs support the survival of marine microalgae in nitrogen-depleted environment
aus: Genome Biology
Abstract Background: Non-cyanobacteria diazotrophs (NCDs) are shown to dominate in surface waters shifting the long-held paradigm of cyanobacteria dominance. This raises fundamental questions on how these putative heterotrophic bacteria thrive in sunlit oceans. The absence of laboratory cultures of these…
London: BioMed Central, 2025-05-28
The Impact of Temperature on Host–Parasite Interactions and Metabolomic Profiles in the Marine Diatom Coscinodiscus granii
Diatoms are single-celled photosynthetic eukaryotes responsible for CO 2 fixation and primary production in aquatic ecosystems. The cosmopolitan marine diatom Coscinodiscus granii can form seasonal blooms in coastal areas and interact with various microorganisms, including the parasitic oomycete Lagenisma…
Basel: MDPI, 2024-12-05
Dimethyl Sulfide Emissions From a Peatland Result More From Organic Matter Degradation Than Sulfate Reduction
Volatile sulfur compounds (VSCs) contribute to acid rain, cloud formation, and albedo, and thus influence the climate. Their global emissions are quite uncertain, especially contributions from freshwater wetlands. We investigated the processes leading to hydrogen sulfide (H 2 S), methanethiol (MeSH),…
Hoboken, NJ: Wiley, 2024-01-05
Reproducible and automated analysis of metabolomics data to assess the biosynthetic capacities of organisms in microbial…
The thesis entitled "Reproducible and automated analysis of metabolomics data to assess the biosynthetic capacities of organisms in microbial communities", investigates the role of marine microbial communities in maintaining ecological balance through the exchange of metabolites. High-throughput omics…
Synchronisierter Zelltod bei der Kieselalge Chaetoceros didymus
Diese Dissertation untersucht den synchronisierten Zelltod bei der Kieselalge Chaetoceros didymus, einem weltweit verbreiteten Mikroalge mit zentraler Bedeutung für marine Ökosysteme. Ziel der Arbeit war es, die Mechanismen hinter dem plötzlichen Absterben von Algenkulturen zu verstehen, die chemischen…
Cardenolide diversity and toxicity in tri-trophic interactions
Natural selection is expected to drive evolution towards a small number of the most beneficial chemical defences. However, in the genus Asclepias there is a high diversity of cardenolides, a compound class that target the Na+/K+—ATPase enzyme. The specialised herbivores of Asclepias are hypothesised…
The role of protein-protein interactions in Bisindole alkaloid biosynthesis in Catharanthus roseus
Diese Arbeit beschreibt detailliert die mechanistische und biophysikalische Charakterisierung von Enzymen, die an der Biosynthese der Gruppe von Verbindungen beteiligt sind, die als Monoterpen-Indol-Alkaloide (MIAs) bekannt sind. Angesichts der pharmakologischen Bedeutung dieser Verbindungen besteht…
Sexual reproduction in pennate diatoms : physiology and chemistry of pheromones active in femtomolar concentration
Chemical communication is the oldest and most widespread form of communication between organisms ranging from unicellular microorganisms to mammals. It includes pheromone signaling for mate choice and is known even from diatoms, a highly diverse group of unicellular photosynthetic organisms. Studying…
MSdeCIpher: A Tool to Link Data from Complementary Ionization Techniques in High-Resolution GC-MS to Identify Molecular Ions
aus: Metabolites
Electron ionization (EI) and molecular ion-generating techniques like chemical ionization (CI) are complementary ionization methods in gas chromatography (GC)-mass spectrometry (MS). However, manual curation effort and expert knowledge are required to correctly assign molecular ions to fragment spectra.…
Basel: MDPI, 2023-12-22
Untargeted metabolomics to expand the chemical space of the marine diatom Skeletonema marinoi
Diatoms (Bacillariophyceae) are aquatic photosynthetic microalgae with an ecological role as primary producers in the aquatic food web. They account substantially for global carbon, nitrogen, and silicon cycling. Elucidating the chemical space of diatoms is crucial to understanding their physiology and…
Lausanne: Frontiers Media S.A., 2023-12-05
Structure Elucidation of the First Sex‐Inducing Pheromone of a Diatom
Abstract Diatoms are abundant unicellular microalgae, responsible for ≈20 % of global photosynthetic CO 2 fixation. Nevertheless, we know little about fundamental aspects of their biology, such as their sexual reproduction. Pheromone‐mediated chemical communication is crucial for successful mating. An…
Weinheim: Wiley, 2023-10-16
Transcriptomics‐guided identification of an algicidal protease of the marine bacterium Kordia algicida OT‐1
aus: MicrobiologyOpen
Abstract In recent years, interest in algicidal bacteria has risen due to their ecological importance and their potential as biotic regulators of harmful algal blooms. Algicidal bacteria shape the plankton communities of the oceans by inhibiting or lysing microalgae and by consuming the released nutrients.…
Malden, Mass.: Wiley, 2023-10-10