In bacteria, regulatory networks controlling the adaptation of gene expression in response to stress are frequently complemented by base-pairing small regulatory RNAs (sRNAs) that act at the post-transcriptional level. While many regulatory circuits governing stress resilience have been studied in the…
Xylella fastidiosa ( Xf ) is a Gram‐negative bacterial plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its virulence is the production of extracellular vesicles (EVs). In this study, we discovered that DNA‐binding proteins and nonribosomal…
the aim of this study was to decipher the transcriptome of the major human pathogen K. pneumoniae and to identify post-transcriptional mechanisms by which this bacterium adapts to changing environmental conditions. The results presented in this thesis substantially expand the understanding of this understudied…
Small RNAs (sRNAs) are non-coding RNAs, that often interact with messenger RNAs (mRNAs) to modulate gene expression. RNA-binding proteins (RBPs) are crucial for stabilizing these interactions. Advances in RNA sequencing (RNA-seq) technologies have enabled the high-throughput characterization of RNA interaction…
Caulobacter crescentus is a key model for studying fundamental prokaryotic processes, including cell cycle progression and homeostasis during stress. While gene expression control has traditionally focused on transcriptional and post-translational levels, recent decades have highlighted an additional…
Severe disease progression and death from influenza virus infections are often linked to secondary infections, such as bacteria or fungi, particularly Aspergillus species. Similarly, the recent pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) resulted in millions of deaths…
Rapid advances in the field of post-transcriptional regulation are uncovering a growing repertoire of mechanisms that modulate the production of functional gene products at the RNA level. Key players in this field are short non-coding RNAs (sRNAs) that form RNA duplexes with target mRNAs, either directly…
Quorum sensing is a microbial signaling process that relies on the production, release, and response to extracellular signaling molecules called autoinducers. This Review covers quorum‐sensing systems controlled by a pyrazine‐type autoinducer, 3,5‐dimethyl‐pyrazin‐2‐ol (DPO). The systems we cover span…
Flagella are multiprotein complexes whose assembly and positioning require complex spatiotemporal control. Flagellar assembly is thought to be controlled by several transcriptional tiers, which are mediated through various master regulators. Here, we revisited the regulation of flagellar genes in polarly…
Small regulatory RNAs (sRNAs) acting in concert with the RNA chaperone Hfq are prevalent in many bacteria and typically act by base-pairing with multiple target transcripts. In the human pathogen Vibrio cholerae , sRNAs play roles in various processes including antibiotic tolerance, competence, and quorum…
Vibrio cholerae , the cause of cholera disease, exhibits a characteristic curved rod morphology, which promotes infectivity and motility in dense hydrogels. Periplasmic protein CrvA determines cell curvature in V. cholerae , yet the regulatory factors controlling CrvA are unknown. Here, we discover the…
To efficiently interact with their environment, bacteria often work in groups to solve complex tasks. Coordination of collective functions requires communication among the members of the group, a process typically referred to as quorum sensing (QS). Marine Vibrio species, including the major human pathogen…
The opportunistic Candida species, C. albicans and C. glabrata, can colonize multiple organs during systemic infection and face different defense activities of the host. Immune cells, like macrophages, ingest fungal cells into phagosomes facing a hostile environment with low nutrient levels. C. albicans…