Introduction Multidrug-resistant (MDR) Klebsiella pneumoniae is a critical healthcare challenge due to its extensive resistance to antibiotics and role in causing severe infections. Bacteriophages offer a promising alternative for targeting MDR pathogens. This study characterizes a novel phage, vbKpUKJ_2,…
Introduction: Accurately assessing biofilm viability is essential for evaluating both biofilm formation and the efficacy of antibacterial treatments. Traditional SYTO9 and propidium iodide (PI) live/dead staining in biofilm viability assays often ace challenges due to non-specific staining, limiting…
Given the rapid cross-country spread of SARS-CoV-2 and the resulting difficulty in tracking lineage spread, we investigated the potential of combining mobile service data and finegranular metadata (such as postal codes and genomic data) to advance integrated genomic surveillance of the pandemic in the…
Nanopore sequencing represents a breakthrough in DNA analysis technology, offering a novel approach to genome sequencing through the passage of DNA molecules through nanopores. This method captures distinctive electrical signals from DNA bases, enabling rapid sequence reconstruction in near real-time.…
Rapid screening of hospital admissions to detect asymptomatic carriers of resistant bacteria can prevent pathogen outbreaks. However, the resulting isolates rarely have their genome sequenced due to cost constraints and long turn-around times to get and process the data, limiting their usefulness to…
Metagenomic sequencing is promising for clinical applications to study microbial composition concerning disease or patient outcomes. Alterations of the vaginal microbiome are associated with adverse pregnancy outcomes, like preterm premature rupture of membranes and preterm birth. Methodologically these…
London: Macmillan Publishers Limited, part of Springer Nature, 2022-03-07
In response to the SARS-CoV-2 pandemic, a highly increased sequencing effort has been established worldwide to track and trace ongoing viral evolution. Technologies, such as nanopore sequencing via the ARTIC protocol are used to reliably generate genomes from raw sequencing data as a crucial base for…