31 Dokumente gefunden

Uneven illumination affects all images acquired by optical microscopes, especially large, multicolour and nonlinear measurements. Although removal is possible with various algorithms, evaluating raw and processed images is challenging due to the lack of established workflows for image quality assessment.…

MINFLUX fluorescence microscopy is a recently introduced super-resolution approach for studying cellular structures and their dynamics with highest detail. MINFLUX (MFX) performs Single Particle Tracking (SPT) at runtime . The ad hoc signal interpretation necessary to sustain the method relies on several…

Lateral diffusion measurements have been ‐used to infer information about the nano‐organization of membranes. We employed interferometric scattering (ISCAT) microscopy at an acquisition rate of 2 kHz to revisit the diffusion dynamics of a phospholipid analog on the plasma membrane of Ptk2 cells. The…

The plasma membrane acts as a dynamic interface between the cell and its environment, where its biophysical properties, such as lipid packing and protein mobility regulate essential cellular processes. These properties are especially vital in epithelial cells, which rely on membrane organization and…

Biologists are always interested in the molecular structures inside living cells, for example, mitochondria. But most of the time, these fine subcellular structures are randomly oriented in X, Y, and Z direction. For faithful image reconstruction, it is always desired to have an isotropic resolution…

In vielen gesundheitsrelevanten Bereichen, wie der Medizin oder der Lebensmittel- und Wasserkontrolle, besteht dringender Bedarf an sensitiven Messmethoden zur schnellen Erkennung von Bakterien und anderen mikrobiellen Keimen. Standardmäßig verwendete Methoden benötigen i.d.R. mehrere Stunden bis zu…

The thesis focuses on the approach of fiber-assisted nanoparticle tracking analysis based on elastic light scattering, clarifies its capability of ultralong and fast-tracking of label-free nanoparticles, points out the limitations in the current schemes, and proposes related solutions. Firstly, the thesis…

Confocal microscopy is well known for its capability to produce 3D images with increased optical resolution and contrast in living multicellular thick samples. Concurrently, it can be used as a powerful spectroscopic tool, facilitating fluorescence methods such as fluorescence correlation spectroscopy…

BODIPY and BODIPY‐derived systems are widely applied as fluorophores and as probes for viscosity detection in solvents and biological media. Their orientational and rotational dynamics in biological media are thus of vital mechanistic importance and extensively investigated. In this contribution, polarization‐resolved…

Abstract Diffusion and mobility are essential for cellular functions, as molecules are usually distributed throughout the cell and have to meet to interact and perform their function. This also involves the cytosolic migration of cellular organelles. However, observing such diffusion and interaction…