29 Dokumente gefunden
The oral cavity as a reservoir for resistance- and hypervirulence-associated genes of Klebsiella pneumoniae in hospitalized…
Introduction: This study investigated the epidemiology and distribution of carbapenem resistance and virulence genes in Klebsiella pneumoniae strains isolated from the oral cavity of hospitalized patients, highlighting their role as reservoirs in non-epidemic contexts. Methods: Carbapenem-resistant Klebsiella…
Lausanne: Frontiers Media S.A., 2026-02-05
Transcriptional Reprogramming of Staphylococcus aureus in Chronic Rhinosinusitis Reveals a Persistence-Associated Phenotype
Chronic rhinosinusitis (CRS) is a persistent inflammatory condition frequently associated with Staphylococcus aureus . The bacterium’s ability to evade immune clearance and establish long-term infection complicates treatment. In our previous study, we demonstrated that S. aureus isolates obtained from…
Basel: Molecular Diversity Preservation International, 2026-01-31
New approaches for molecular typing in Gram-positive pathogens
Antimicrobial resistance (AMR) represents one of the most severe global health threats of the 21st century. Vancomycin-resistant enterococci (VRE), primarily Enterococcus faecium and E. faecalis, are significant nosocomial pathogens that cause difficult-to-treat infections with high mortality rates in…
Third-Generation Cephalosporin-Resistant Enterobacterales and Methicillin-Resistant Staphylococcus aureus (MRSA) in Pigs…
aus: Animals
Simple Summary This study explored the presence of antibiotic-resistant bacteria in pigs and their surroundings on farms in Rwanda. Antibiotic resistance occurs when bacteria change and become harder to kill with medicines, which is a serious problem for both human and animal health worldwide. We collected…
Basel: MDPI, 2025-12-31
Culture-Based Wastewater Surveillance for the Detection and Monitoring of Antimicrobial Resistance in Staphylococcal Species
aus: Veterinary Sciences
Simple Summary Antimicrobial resistance, when bacteria change in ways that make antibiotics no longer effective, has become one of the most serious global healthcare challenges of our time. This study examined bacteria found in wastewater in Dubai to better understand how resistant bacteria spread across…
Basel: MDPI, 2025-12-23
Genomic characterization of Staphylococcus aureus isolated from mastitis in small ruminants in Sardinia, Italy
Background Staphylococcal mastitis is a common disease of small ruminants causing major economic losses. The problem is particularly significant in the rural areas of the Mediterranean region, where almost two thirds of the global sheep and a quarter of the global goat milk are produced. This study aimed…
Lausanne: Frontiers Media S.A., 2025-12-02
Characterization of Staphylococcus aureus CC1 and CC1660 of Human and Equine Origin
aus: Antibiotics
Background/Objectives : Staphylococcus aureus isolates from humans and horses of the equine-associated clonal complexes (CCs) CC1 and CC1660 were comparatively investigated for their genomic relationships. Methods : A total of 91 S. aureus isolates (64 human, 27 equine) were subjected to whole-genome…
Basel: MDPI, 2025-10-27
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform
Introduction Carbapenemase-producing bacteria undermine the efficacy of carbapenems, a class of last-resort antibiotics used primarily to treat infections caused by multidrug-resistant Gram-negative pathogens. Carbapenemases are among the most alarming antimicrobial resistance mechanisms because they…
Lausanne: Frontiers Media S.A., 2025-08-26
Rapid Detection of Panton–Valentine Leukocidin Production in Clinical Isolates of Staphylococcus aureus from Saxony and Brandenburg…
aus: Pathogens
Panton–Valentine leukocidin (PVL) is a staphylococcal toxin associated with chronic/recurrent skin and soft tissue infections (SSTIs) and necrotizing pneumonia. Its detection in clinical isolates of Staphylococcus aureus warrants aggressive therapy and infection control measures. However, PVL detection…
Basel: MDPI, 2025-03-01
Rapid Simultaneous Detection of the Clinically Relevant Carbapenemase Resistance Genes bla KPC, bla OXA48, bla VIM and bla…
Antibiotic resistance, in particular the dissemination of carbapenemase-producing organisms, poses a significant threat to global healthcare. This study introduces the qPCR CarbaScan LyoBead assay, a robust, accurate, and efficient tool for detecting key carbapenemase genes, including bla KPC, bla NDM,…
Basel: Molecular Diversity Preservation International, 2025-01-30
Methicillin-Resistant Staphylococcus aureus : The Shifting Landscape in the United Arab Emirates
aus: Antibiotics
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant burden globally, particularly in the Arabian Gulf region. The United Arab Emirates (UAE) has experienced rising MRSA prevalence, with increasing diversity in the clonal complexes (CCs) identified. The COVID-19 pandemic, with…
Basel: MDPI, 2025-01-02
Tracking Multidrug Resistance in Gram-Negative Bacteria in Alexandria, Egypt (2020–2023): An Integrated Analysis of Patient…
aus: Antibiotics
Background: The rise in carbapenem-resistant Enterobacteriaceae (CRE) in Egypt, particularly in hospital settings, poses a significant public health challenge. This study aims to develop a combined epidemiological surveillance tool utilizing the Microreact online platform (version 269) and molecular…
Basel: MDPI, 2024-12-05
Physik und Bakterien
aus: Öffentliche Samstagsvorlesung der Physikalisch-Astronomischen Fakultät
Wintersemester 2024/2025
Diese Vorlesung vom Mikrobiologen Ralf Ehricht und Biophysiker Christian Eggeling gibt einen Einblick in die Welt der Bakterien und wie wir diese physikalisch beschreiben und beobachten können. Bakterien kommen überall vor und unser Körper ist gefüllt davon und sie sind auch essentiell für viele Funktionen.…
Jena: Friedrich-Schiller-Universität, 2024-11-02
Fast and Economic Microarray-Based Detection of Species-, Resistance-, and Virulence-Associated Genes in Clinical Strains…
aus: Sensors
Today, there is a continuous worldwide battle against antimicrobial resistance (AMR) and that includes vancomycin-resistant enterococci (VRE). Methods that can adequately and quickly detect transmission chains in outbreaks are needed to trace and manage this problem fast and cost-effectively. In this…
Basel: MDPI, 2024-10-08
Monitoring the Dilution of Buffer Solutions with Different pH Values above and below Physiological pH in Very Small Volumes
aus: Sensors
The accurate determination of the post-dilution concentration of biological buffers is essential for retaining the necessary properties and effectiveness of the buffer to maintain stable cellular environments and optimal conditions for biochemical reactions. In this work, we introduce a silicon-based…
Basel: MDPI, 2024-09-04
Imaging Diffractometric Biosensors for Label-Free, Multi-Molecular Interaction Analysis
Biosensors are used for the specific and sensitive detection of biomolecules. In conventional approaches, the suspected target molecules are bound to selected capture molecules and successful binding is indicated by additional labelling to enable optical readout. This labelling requires additional processing…
Basel: MDPI, 2024-08-17
A Proof-of-Concept Protein Microarray-Based Approach for Serotyping of Salmonella enterica Strains
aus: Pathogens
Salmonella enterica , a bacterium causing foodborne illnesses like salmonellosis, is prevalent in Europe and globally. It is found in food, water, and soil, leading to symptoms like diarrhea and fever. Annually, it results in about 95 million cases worldwide, with increasing antibiotic resistance posing…
Basel: MDPI, 2024-04-25
Reduced Glycolysis and Cytotoxicity in Staphylococcus aureus Isolates from Chronic Rhinosinusitis as Strategies for Host…
Chronic rhinosinusitis (CRS) is a multifactorial infection of the nasal cavity and sinuses. In this study, nasal swabs from control donors (N = 128) and patients with CRS (N = 246) were analysed. Culture methods and metagenomics revealed no obvious differences in the composition of the bacterial communities…
Basel: Molecular Diversity Preservation International, 2024-02-13
Nanopore-Sequenzierung und automatisierte bioinformatische Datenanalyse für innovative mikrobiologische Diagnostik
Nanopore sequencing represents a breakthrough in DNA analysis technology, offering a novel approach to genome sequencing through the passage of DNA molecules through nanopores. This method captures distinctive electrical signals from DNA bases, enabling rapid sequence reconstruction in near real-time.…
A Novel Approach to Monitor the Concentration of Phosphate Buffers in the Range of 1 M to 0.1 M Using a Silicon-Based Impedance…
We present a novel and easy approach using a silicon-based impedance chip to determine the concentration of the given aqueous buffer solution. An accurate determination of the post-dilution concentration of the buffers is necessary for ensuring optimal buffer capacity, pH stability, and to assess solution…
Basel: MDPI, 2023-08-24