24 Dokumente gefunden

Biphasic parameter determination of sheep articular cartilage from stress relaxation data using an optimized 3D FE-based…

Abstract Mechanical characteristics of hard and soft tissues are central features for the quantitative description of tissue properties. In this study, we present a biphasic 3D-FE-based method to determine the biomechanical properties of sheep stifle joint articular cartilage (load-bearing area of the…
Berlin: De Gruyter, 2023-09-01

BMP-2 (and partially GDF-5) coating significantly accelerates and augments bone formation close to hydroxyapatite/tricalcium-phosphate/brushite…

Abstract Bilateral defects (diameter 8 mm) in the medial tibial head of senile, osteopenic female sheep ( n  = 48; 9.63 ± 0.10 years; mean ± SEM) were treated with hydroxyapatite (HA)/beta-tricalcium phosphate (β-TCP)/dicalcium phosphate dihydrate (DCPD; brushite) cylinders coated with BMP-2 (25 or 250…
Erschienen: Springer US, 2023-06-28

Single Application of Low-Dose, Hydroxyapatite-Bound BMP-2 or GDF-5 Induces Long-Term Bone Formation and Biomechanical Stabilization…

Effects of hydroxyapatite (HA) particles with bone morphogenetic BMP-2 or GDF-5 were compared in sheep lumbar osteopenia; in vitro release in phosphate-buffered saline (PBS) or sheep serum was assessed by ELISA. Lumbar (L) vertebral bone defects (Ø 3.5 mm) were generated in aged, osteopenic female sheep…
Basel: MDPI, 2022-02-21

In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model

Resorbable polyglycolic acid (PGA) chondrocyte grafts are clinically established for human articular cartilage defects. Long-term implant performance was addressed in a standardized in vitro model. PGA implants (+/− bovine chondrocytes) were placed inside cartilage rings punched out of bovine femoral…
Basel: Molecular Diversity Preservation International, 2021-10-29

A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts

Mechanotransduction is elicited in cells upon the perception of physical forces transmitted via the extracellular matrix in their surroundings and results in signaling events that impact cellular functions. This physiological process is a prerequisite for maintaining the integrity of diarthrodial joints,…
Basel: MDPI, 2021-10-09

Performance of Calcium Phosphate Cements in the Augmentation of Sheep Vertebrae : An Ex Vivo Study

aus: Materials
Oil-based calcium phosphate cement (Paste-CPC) shows not only prolonged shelf life and injection times, but also improved cohesion and reproducibility during application, while retaining the advantages of fast setting, mechanical strength, and biocompatibility. In addition, poly(L-lactideco-glycolide)…
Basel: MDPI, 2021-07-12

The inverse spacer - A novel, safe, and cost-effective approach in routine procedures for revision knee arthroplasty

Background: A major disadvantage of current spacers for two-stage revision total knee arthroplasty (R-TKA) is the risk of (sub-) luxation during mobilization in the prosthesis-free interval, limiting their clinical success with detrimental consequences for the patient. The present study introduces a…
Basel: MDPI, 2021-03-02

Thickness of the Stifle Joint Articular Cartilage in Different Large Animal Models of Cartilage Repair and Regeneration

aus: CARTILAGE
Objective Regulatory guidelines for preclinical cartilage repair studies suggest large animal models (e.g., sheep, goat, [mini]-pig, or horse) to obtain results representative for humans. However, information about the 3-dimensional thickness of articular cartilage at different implantation sites in…
Sage CA: Los Angeles, CA: SAGE Publications, 2020-12-03

Association of Human FOS Promoter Variants with the Occurrence of Knee-Osteoarthritis in a Case Control Association Study

Our aim was to analyse (i) the presence of single nucleotide polymorphisms (SNPs) in the JUN and FOS core promoters in patients with rheumatoid arthritis (RA), knee-osteoarthritis (OA), and normal controls (NC); (ii) their functional influence on JUN/FOS transcription levels; and (iii) their associations…
Basel: Molecular Diversity Preservation International, 2019-03-19

In vitro und in vivo Testung von Knorpelersatzmaterialien für chonrale und osteochondrale Defekte im Knie

Hyaliner Gelenkknorpel ist frei von Nerven, Blut und Lymphgefäßen und besitzt dadurch nur eine begrenzte Regenerationsfähigkeit. Die Zufuhr der Nährstoffe erfolgt ausschließlich per Diffusion durch die extrazelluläre Matrix (EZM) und/oder die Synovialflüssigkeit. Nicht behandelte Knorpeldefekte können…

Biotechnologically modified calcium phosphate cement for the stabilization of osteoporotic vertebral compression fractures

Osteoporosis is characterized by reduced bone mass and disruption of bone architecture, resulting in an increased risk of fragility fractures, most of which are located in the lumbar spine. Bioresorbable calcium phosphate cement (CPC), mechanically stabilized by the addition of poly(l-lactide-co-glycolide)…

Effect of bioinspired surface microstructures on hemodynamics and platelet adhesion

Occlusion by thrombosis due to the absence of the endothelial cell (EC) layer is one of the most frequent causes of failure of artificial vascular grafts. Bioinspired surface structures may have a potential to reduce the adhesion of platelets contributing to hemostasis. The aim of this PhD research was…

Die Bedeutung von Prostaglandin E2-Rezeptoren für die TNF-α-induzierten pro-inflammatorischen / pro-destruktiven Funktionen…

Die rheumatoide Arthritis (RA) ist eine Erkrankung von erheblicher gesellschaftlicher und volkswirtschaftlicher Relevanz. Für die zukünftige Erforschung neuer therapeutischer Ansätze ist ein Verständnis der Pathogenese der RA unerlässlich. Die vorliegende Arbeit will dazu einen Beitrag leisten, indem…

Expression von Wachstumsfaktoren (z.B. bone morphogenic proteins), Matrixmoekülen und Entzündungsmediatoren in der Bursa…

Periartikuläre Schultererkrankungen haben in den Industrienationen eine hohe Prävalenz (6,6 – 25%) und führen wegen der Begleitschmerzen und Bewegungseinschränkungen zu einem hohen Leidensdruck. Zu diesen Schultererkrankungen zählen in absteigender Häufigkeit Rupturen der Rotatorenmanschette (RM), Impingementsyndrom,…

Integrative methods for reconstruction of dynamic networks in chondrogenesis

Application of human mesenchymal stem cells represents a promising approach in the field of regenerative medicine. Specific stimulation can give rise to chondrocytes, osteocytes or adipocytes. Investigation of the underlying biological processes which induce the observed cellular differentiation is essential…

Integrative modeling of transcriptional regulation in response to autoimmune desease therapies

Die rheumatoide Arthritis (RA) und die Multiple Sklerose (MS) werden allgemein als Autoimmunkrankheiten eingestuft. Zur Behandlung dieser Krankheiten werden immunmodulatorische Medikamente eingesetzt, etwa TNF-alpha-Blocker (z.B. Etanercept) im Falle der RA und IFN-beta-Präparate (z.B. Betaferon und…

Untersuchungen zu biologischen Markern des Skelettmetabolismus in der Synovialflüssigkeit bei Rheumatoider Arthritis

Zielstellung: Ziel der vorliegenden Arbeit war es, verschiedene Marker des Skelettmetabolismus (SM) in der Synovialflüssigkeit bei Rheumatoider Arthritis (RA) in Abhängigkeit von entzündlicher Aktivität, Röntgenstadium und Geschlecht zu untersuchen, sowie deren Beziehungen untereinander nachzuweisen.…

Der Einfluss von TNF-α und IL-1β auf die Knorpeldestruktion durch dynoviale Fibroblasten : Untersuchungen in einem in vitro-Knorpeldestrutkions-Assay…

Die rheumatoide Arthritis (RA) ist eine chronisch-entzündliche Systemerkrankung mit charakteristischer Hyperplasie der Synovialmembran und Zerstörung der befallenen Gelenke. Hierbei wird den aktivierten synovialen Fibroblasten (SFB) eine besondere pathogenetische Bedeutung zugeschrieben, da sie durch…