28 Dokumente gefunden
PRIAMOS: A technique for mixing embedding media for freely adjusting pH value and refractive index (RI) for optical clearing…
Abstract Investigations of biological samples often require sample transparency, which is achieved by embedding the sample in a high‐refractive index (RI) medium to obtain a homogenous RI distribution in the sample, referred to as optical clearing (OC). Here, we introduce a method for designing embedding…
Oxford: Wiley-Blackwell; Soc.; Blackwell, 2025-11-21
Nanochemical Cell-Surface Evaluation in Photothermal Spectroscopic Imaging of Antimicrobial Interactions in the Model System…
aus: Analytical Chemistry
The power of photothermal spectroscopic imaging to visualize antimicrobial interactions on the surfaces of individual bacteria cells has been demonstrated on the model system Bacillus subtilis and vancomycin using mid-infrared photoinduced force microscopy (PiF-IR, also mid-IR PiFM). High-resolution…
Columbus, Ohio: American Chemical Society, 2025-10-24
Time-deterministic cryo-optical microscopy
Fluorescence microscopy enables the visualization of cellular morphology, molecular distribution, ion distribution, and their dynamic behaviors during biological processes. Enhancing the signal-to-noise ratio (SNR) in fluorescence imaging improves the quantification accuracy and spatial resolution; however,…
London: Nature Publishing Grou, 2025-08-23
Combining image scanning and stimulated emission depletion microscopy to achieve isotropic resolution
Biologists are always interested in the molecular structures inside living cells, for example, mitochondria. But most of the time, these fine subcellular structures are randomly oriented in X, Y, and Z direction. For faithful image reconstruction, it is always desired to have an isotropic resolution…
Reconstruction of subcellular structures from resolution-limited volume electron microscopy
Volume electron microscopy (vEM) of biological samples achieves high resolution with generic contrast, providing access to the intricate 3D organization of tissues and cells. It has been used extensively for connectomics—the reconstruction of biological nervous systems—but also serves as a powerful tool…
Theory of computing point spread functions
Das Bildgebungsverfahren mit inkohärentem Licht in der Fluoreszenzmikroskopie ist mathematisch formuliert als Faltung eines Objekts mit der optischen Antwort des Systems, der Punktspreizfunktion (PSF). Diese Antwort findet unter bestimmten Bildgebunsbedingungen, wie z.B. der Verschiebungsinvarianz und…
Image processing for correlative light and electron microscopy
People have never stopped exploring the microscopic world. Studying the microstructure of cells helps people better understand the people themselves and has the potential to overcome specific diseases at a fundamental level. Correlative light and electron microscopy (CLEM) can let people intuitively…
Chiral structured illumination microscopy : a microscopy modality for imaging fluorescent chiral domains at sub-wavelength…
This thesis covers the development of chiral structured illumination microscopy (chiral SIM), a novel wide-field super-resolution microscopy that is able to address the shortcomings restricting the capability of conventional chiral imaging techniques. Ac- cordingly, the research background and fundamentals…
Modular Open-Source toolbox for optics education
UC2 is an open-source modular toolbox for making hardware projects based on functional optical blocks. It creates a bridge between education and science by offering an alternative to the standard tools, a generic toolbox that can be used for both fields. The same basic hardware is meant to be used for…
Holistic improvement of image acquisition and reconstruction in fluorescence microscopy
Recent developments in microscopic imaging led to a better understanding of intra- and intercellular metabolic processes and, for example, to visualize structural properties of viral pathogens. In this thesis, the imaging process of widefield and confocal scanning microscopy techniques is treated holistically…
Light sheet integral field raman microspectroscopy
State of the art biological Raman microscopy currently faces significant drawbacks of low imaging acquisition rate and low signal-to-noise-ratio (SNR). This thesis aims to overcome those issues, introducing an alternative Raman imaging technique. This method is based on light sheet microscopy combined…
PEGylation of Guanidinium and Indole Bearing Poly(methacrylamide)s – Biocompatible Terpolymers for pDNA Delivery
This study describes the first example for shielding of a high performing terpolymer that consists of N ‐(2‐hydroxypropyl)methacrylamide (HPMA), N ‐(3‐guanidinopropyl)methacrylamide (GPMA), and N ‐(2‐indolethyl)methacrylamide monomers (IEMA) by block copolymerization of a polyethylene glycol derivative…
Weinheim: Wiley-VCH, 2021-10-18
Democratizing microscopy by introducing innovative Open-Source hard and software tools
Science lives from the curiosity to get to the bottom of problems and from the subsequent discussion, where scientists exchange knowledge and opinions to finally come up with new questions. However, as a recent study showed, the vast majority of the experiments conducted within publications can often…
Improving the signal-to-noise ratio in incoherent imaging
Imaging plays an important role in our modern world. Besides limitations in terms of resolution, all imaging systems show degrading image quality with increasing noise. Hence, an important aspect of any imaging application is the achievable signal-to-noise ratio (SNR). When neglecting typical noise sources…
Advanced single molecule localization microscopy for imaging cellular nuclei
In this PhD research, single molecule localization microscopy (SMLM) was used to image nuclear structures with a resolution down to several nanometers.The scope of this PhD research is to develop a 3D SMLM microscope which can overcome several principle limitations in imaging nuclei in 3D. The advanced…
Methods and instrumentation for raman characterization of bladder cancer tumor
High incidence and recurrence rates make bladder cancer the most common malignant tumor in the urinary system. Cystoscopy is the gold standard test used for diagnosis, nevertheless small flat tumors might be missed, and the procedure still represents discomfort to patients and high recurrence can result…
High-resolution direct stochastic optical reconstruction microscopy of the human kinetochore chromatin
<!--StartFragment -->The kinetochore is a chromatin-protein complex on the chromosome centromere. Kinetochore attache chromosomes to the microtubules of the spindle apparatus, recognize attachment to microtubules, and regulate the progress of mitosis. The kinetochore protein complex is built on the periphery…
Towards clinical translation of raman spectroscopy for tumor cell identification
In the modern world, cancer is one of the leading causes of death, and its early diagnostics remains one of the big challenges. Since cancer starts as a malfunction on the cellular level, the diagnostic techniques have to deal with single cells. Detection of circulating tumor cells (CTCs), which are…
Uptake and intracellular fate of polymeric nanoparticles studied by light and electron microscopy : from labeling strategies…
In nanomedicine, synthetic nanoscale objects are utilized as vectors to transfer pharmaceutically active compounds into partiular cell types, where they are released in a controlled manner to address intracellular organelles selectively. Polymeric nanomaterials, which represent a material class of tremendous…