GABA A receptors are ligand-gated ion channels, which are predominantly permeable for chloride. The neuronal K-Cl cotransporter KCC2 lowers the intraneuronal chloride concentration and thus plays an important role for GABA signaling. KCC2 loss-of-function is associated with seizures and epilepsy. Here,…
Lausanne: Frontiers Research Foundation, 2022-02-03
In this thesis the NanoString nCounter technology was applied to validate an improved PCR-based strategy for quantitative and qualitative global single cell transcriptome analysis. RNA isolates from whole tissue were quantitatively compared with cDNA amplificates prepared from single cell equivalent…