000K utf8 1100 $c2025 1500 eng 2050 urn:nbn:de:gbv:27-dbt-69373-0 2051 10.1126/sciadv.ads6417 3000 Furch, Alexandra C. U. 3010 Buxa-Kleeberg, Stefanie V 3010 Ehlers, Katrin 3010 Fliegmann, Judith 3010 Grabe, Veit 3010 Hafke, Jens B 3010 Haufschild, Tom 3010 Koch, Aline M 3010 Kogel, Karl-Heinz 3010 Maaß, Jan-Peter 3010 Mithöfer, Axel 3010 Noll, Gundula A 3010 Nötzold, Jonas 3010 Oelmüller, Ralf 3010 Peiter, Edgar 3010 Prüfer, Dirk 3010 Scholz, Sandra S. 3010 Teutemacher, Florian 3010 van Bel, Aart J E 3010 Wrobel, Lisa S 3010 Zimmermann, Matthias R. 4000 Transformation of flg22 perception into electrical signals decoded in vasculature leads to sieve tube blockage and pathogen resistance$hAmerican Association for the Advancement of Science (AAAS) [Furch, Alexandra C. U.] 4030 $nAmerican Association for the Advancement of Science (AAAS) 4060 23 Seiten 4209 This study focuses on the question how and where information acquired by FLS2 perception of flg22 is transformed into electrical signals crucial for generation of local and systemic defense responses. In Arabidopsis thaliana and Vicia faba leaves, FLS2 density was high in the epidermis and vascular parenchyma, low in mesophyll, and absent in sieve elements (SEs). Aequorin-based examinations disclosed dual cytosolic Ca2+ peaks shortly after flg22 application, which corresponded with two voltage shifts from the epidermis to SEs. These signals were converted into rapid long-range action potentials (APs) or slower short-range variation potentials (VPs). Modified phytohormone-levels demonstrated systemic AP effects. Jasmonic acid up-regulation was significantly higher in wild-type than Atseor1/2 mutants. Abundant Ca2+ influx associated with VPs was responsible for transient sieve element occlusion (SEO) near the flg22 perception site, whereas SEO was absent in Atseor1/2 and Atfls2 mutants. Biological relevance of SEO was demonstrated by higher susceptibility of Atseor1/2 mutants to Pseudomonas syringae than wild-type plants. 4950 https://doi.org/10.1126/sciadv.ads6417$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:27-dbt-69373-0$xR$3Volltext$534 4961 https://www.db-thueringen.de/receive/dbt_mods_00069373 5051 570 5550 Arabidopsis 5550 Arabidopsis Proteins 5550 Calcium 5550 Cyclopentanes 5550 Disease Resistance 5550 Flagellin 5550 Gene Expression Regulation, Plant 5550 Mutation 5550 Oxylipins 5550 Plant Diseases 5550 Plant Leaves 5550 Pseudomonas syringae 5550 Signal Transduction 5550 Vicia faba