https://www.db-thueringen.de/receive/dbt_mods_00068541https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00069927/42003_2025_Article_9060.pdfhttps://www.db-thueringen.de/rsc/thumbnail/dbt_mods_00068541.png text article article Parameter optimization for MINFLUX microscopy enabled single particle tracking Vogler, Bela T. L. Vogler Bela T. L. Institute of Applied Optics and Biophysics Friedrich-Schiller University Jena Jena Germany Department of Biophysical Imaging, Leibniz Institute of Photonic Technologies e.V. Jena Germany Leibniz Center for Photonics in Infection Research (LPI) Jena Germany aut Author 0000-0002-5598-5738 1330291042 urn:nbn:de:gbv:27-dbt-68541-0 De Angelis, Giovanni De Angelis Giovanni Institute of Applied Optics and Biophysics Friedrich-Schiller University Jena Jena Germany Department of Biophysical Imaging, Leibniz Institute of Photonic Technologies e.V. Jena Germany aut Author 1358653755 Zhao, Ziliang Zhao Ziliang Institute of Applied Optics and Biophysics Friedrich-Schiller University Jena Jena Germany Department of Biophysical Imaging, Leibniz Institute of Photonic Technologies e.V. Jena Germany Leibniz Center for Photonics in Infection Research (LPI) Jena Germany aut Author 1277572631 Eggeling, Christian Eggeling Christian Institute of Applied Optics and Biophysics Friedrich-Schiller University Jena Jena Germany Department of Biophysical Imaging, Leibniz Institute of Photonic Technologies e.V. Jena Germany Leibniz Center for Photonics in Infection Research (LPI) Jena Germany Jena Center for Soft Matter Jena Germany aut Author 0000-0002-3698-5599 122804120 Reina, Francesco Reina Francesco Institute of Applied Optics and Biophysics Friedrich-Schiller University Jena Jena Germany Department of Biophysical Imaging, Leibniz Institute of Photonic Technologies e.V. Jena Germany Leibniz Center for Photonics in Infection Research (LPI) Jena Germany Max Perutz Labs, Department of Structural and Computational Biology University of Vienna Vienna Austria aut Author 0000-0001-6752-9089 1330296907 10.1038/s42003-025-09060-1 2025-11-14 8 1 1573 1 12 continuingjournal journal Communications Biology 2399-3642 Springer Nature 2018 London text en 570 MINFLUX fluorescence microscopy is a recently introduced super-resolution approach for studying cellular structures and their dynamics with highest detail. MINFLUX (MFX) performs Single Particle Tracking (SPT) at runtime . The ad hoc signal interpretation necessary to sustain the method relies on several parameters, which need to be optimized in relation to the sample under study, such as fluorescent lipid analogs in membranes, to ensure the fidelity of the measurement. We propose a parameter optimization strategy, give an overview of the most important parameters, present a theoretical upper limit for trackable diffusion rates, and demonstrate MFX -enabled SPT of fast (⟨DMSD⟩ = 2.5 μm²/s) lateral Brownian motion of lipids in membranes. en 12 Seiten 530 his Host institution bibliography Zweitveröffentlichung dbt_mods_00068541https://www.db-thueringen.de/receive/dbt_mods_00068541