PT Journal
AU Vogler, B
   De Angelis, G
   Zhao, Z
   Eggeling, C
   Reina, F
TI Parameter optimization for MINFLUX microscopy enabled single particle tracking
SO Communications Biology
PD November
PY 2025
BP 1
EP 12
VL 8
IS 1
PU Springer Nature
DI 10.1038/s42003-025-09060-1
WP https://www.db-thueringen.de/receive/dbt_mods_00068541
LA en
SN 2399-3642
AB MINFLUX fluorescence microscopy is a recently introduced super-resolution approach for studying cellular structures and their dynamics with highest detail. MINFLUX (MFX) performs Single Particle Tracking (SPT) at runtime . The ad hoc signal interpretation necessary to sustain the method relies on several parameters, which need to be optimized in relation to the sample under study, such as fluorescent lipid analogs in membranes, to ensure the fidelity of the measurement. We propose a parameter optimization strategy, give an overview of the most important parameters, present a theoretical upper limit for trackable diffusion rates, and demonstrate MFX -enabled SPT of fast (⟨DMSD⟩ = 2.5 μm²/s) lateral Brownian motion of lipids in membranes.
PI London
ER