https://www.db-thueringen.de/receive/dbt_mods_00068520https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00069907/10753_2025_Article_2269.pdfhttps://www.db-thueringen.de/rsc/thumbnail/dbt_mods_00068520.png text article The Vitamin E Derivative Garcinoic Acid Suppresses NLRP3 Inflammasome Activation and Pyroptosis in Murine Macrophages Börmel, Lisa Börmel Lisa Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 0000-0001-8169-5913 1217018379 article Geisler, Anja R. Geisler Anja R. Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 0009-0000-9752-713X 1364701561 Hupfer, Yvonne Hupfer Yvonne Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 1355279925 Liao, Sijia Liao Sijia Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 0000-0003-0844-3909 1325154342 Schubert, Tina Schubert Tina Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 134333151X urn:nbn:de:gbv:27-dbt-68520-5 Kluge, Stefan Kluge Stefan Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 1216652511 Lorkowski, Stefan Lorkowski Stefan Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 0000-0002-9649-840X 12292620X Wallert, Maria Wallert Maria Institute of Nutritional Science, Friedrich Schiller University 07743 Jena Germany aut Author 0000-0003-3796-0729 1172048932 10.1007/s10753-025-02269-6 2025-10 48 5 3340 3352 continuingjournal journal Inflammation International Inflammation Research Society 0360-3997 1573-2576 Springer US 1975 New York, NY text en 610 Excessive inflammation in cells are a common cause of inflammation-related diseases such as cardiometabolic diseases. The cellular multiprotein complex nucleotide-binding domain and leucine-rich repeat pyrin domain 3 (NLRP3) inflammasome is a cellular key modulator of inflammatory processes. In addition to classic medications, phytochemicals are known for their anti-inflammatory potential. In African folk medicine the seeds of Garcinia kola are used to support the treatment of inflammatory diseases. Of particular interest is the phytochemical garcinoic acid (GA, trans -13′-carboxy-δ-tocotrienol), which is isolated from the Garcinia kola seeds. This derivative and potential metabolite of the vitamin E congener δ-tocotrienol (T3) shows anti-inflammatory properties in vitro. However, the underlying mechanisms are largely unknown. To get better insights into the molecular mode of action, murine J774A.1 macrophages were stimulated with lipopolysaccharides (LPS) only or in combination with adenosine triphosphate (ATP), which led to canonical activation of the NLRP3 inflammasome and subsequent pyroptosis. A combined treatment with GA resulted in significantly reduced stimulation of the transcription factor nuclear factor 'ĸ-light-chain-enhancer' of activated B-cells (NF-ĸB), decreased expression of inflammasome-related genes and marked downregulation of autoproteolytic cleavage of caspase-1 (Casp-1). Consequently, GA had an inhibitory effect on pyroptosis. The results have been validated using the well-known NLRP3 inflammasome inhibitor MCC950. In conclusion, GA was shown to have relevant effects on the regulation of the NLRP3 inflammasome and pyroptosis in vitro. Our study provides new mechanistic insights into the anti-inflammatory mode of action of GA and highlights its relevance as a potential phytochemical drug for the treatment of inflammation. NLRP3 inflammasome Pyroptosis Macrophages Vitamin E Garcinoic acid en 13 Seiten 570 his Host institution bibliography Zweitveröffentlichung dbt_mods_00068520https://www.db-thueringen.de/receive/dbt_mods_00068520