@PhdThesis{dbt_mods_00068164,
  author = 	{Velasco Gomariz, Manuel},
  title = 	{Deciphering the RNA interactome of Caulobacter crescentus},
  year = 	{2025},
  address = 	{Jena},
  keywords = 	{Small RNA; Caulobacter crescentus},
  abstract = 	{Caulobacter crescentus is a key model for studying fundamental prokaryotic processes, including cell cycle progression and homeostasis during stress. While gene expression control has traditionally focused on transcriptional and post-translational levels, recent decades have highlighted an additional regulatory layer: small regulatory RNAs (sRNAs). These molecules are pivotal in modulating transcript stability and translation via direct, often imperfect base pairing. Currently, less than a dozen sRNAs have been described in Caulobacter, significantly fewer than in other model organisms. This work describes the application of two strategies to illuminate sRNA-mediated regulation in Caulobacter. CauloSOEP, a multi-copy plasmid library, was used to systematically study the activities of 50 C. crescentus sRNAs. The overexpression of one sRNA, AbnZ, impaired cell viability and altered cell morphology. In-depth characterization of AbnZ, which is processed from the 3' end of the abn mRNA, revealed that it represses the essential Translocation and Assembly Module (TAM) system. The RIL-seq (RNA interaction by ligation and sequencing) technology was applied in a global approach to map the RNA-RNA interactome associated with the RNA chaperone Hfq. This mapping identified hundreds of previously undetected Hfq-dependent RNA-RNA interactions, including unconventional pairings between two sRNAs. Notably, the sRNA CrfA acts as an RNA sponge to sequester other negatively acting sRNAs, thereby indirectly promoting target gene expression. CrfA is specifically required in response to carbon starvation to rewire the Caulobacter transcriptome and aid in cellular adaptation to stress. These findings underscore the critical role of sRNAs in the regulatory architecture of C. crescentus.},
  note = 	{Dissertation, Friedrich-Schiller-Universit{\"a}t Jena, 2025},
  doi = 	{10.22032/dbt.68164},
  url = 	{https://www.db-thueringen.de/receive/dbt_mods_00068164},
  url = 	{http://uri.gbv.de/document/gvk:ppn:1940193338},
  url = 	{https://doi.org/10.22032/dbt.68164},
  file = 	{:https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00069599/Dissertation_ManuelVelascoGomariz.pdf:PDF},
  language = 	{en}
}