000K utf8 0100 1939392470 1100 $c2025 1500 eng 2050 urn:nbn:de:gbv:27-dbt-67820-5 2051 10.22032/dbt.67820 3000 Lippegaus, Anne 4000 RNA networks controlling collective behaviors in Vibrio cholerae [Lippegaus, Anne] 4060 201 Seiten 4209 Small RNAs (sRNAs) are non-coding RNAs, that often interact with messenger RNAs (mRNAs) to modulate gene expression. RNA-binding proteins (RBPs) are crucial for stabilizing these interactions. Advances in RNA sequencing (RNA-seq) technologies have enabled the high-throughput characterization of RNA interaction partners, providing insights into RNA networks on a transcriptome-wide scale. Global methods, such as RIL-seq (RNA interaction by ligation and sequencing), have been developed to study RNA interactomes in vivo. This study employs RIL-seq with Hfq in Vibrio cholerae at low cell density (LCD) and high cell density (HCD). Thereby we identified 2,889 statistically significant RNA-RNA interactions. A deeper analysis revealed a previously uncharacterized RNA sponge, QrrX, which specifically interacts with the Quorum regulatory RNAs 1-4 (Qrr1-4). Moreover, this study presents an interactive RIL-seq browser to facilitate the identification of chimeric RNA molecules, derived from high-throughput methods. Among our findings, we identified two novel sRNAs, NetX and NetY (network-derived sRNAs X and Y), and demonstrated that NetX is a previously unrecognized regulator of virulence gene expression in V. cholerae. Furthermore, we investigated post-transcriptional regulation of virulence factors in V. cholerae. RNA-seq and RIL-seq analyses were conducted under virulence-inducing conditions, leading to the identification of a novel virulence-related sRNA. We named this sRNA CisR (CTXφ inhibiting sRNA) due to its regulatory effect on the cep mRNA, which is encoded by the filamentous bacteriophage CTXφ. Collectively, the findings presented in this thesis reveal the complexity of the sRNA landscape in V. cholerae and provides an interactive visualization of RNA interactomes in V. cholerae under both standard laboratory conditions and virulence inducing conditions and thus lays the foundation for discovering new mechanisms of RNA-based regulation. 4950 https://doi.org/10.22032/dbt.67820$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:27-dbt-67820-5$xR$3Volltext$534 4961 http://uri.gbv.de/document/gvk:ppn:1939392470 5051 570 5550 RNS 5550 Small RNA 5550 Vibrio cholerae 5550 Virulenz