https://www.db-thueringen.de/receive/dbt_mods_00067076https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00068626/1999-4923_15_2023_11_2616.pdfhttps://www.db-thueringen.de/rsc/thumbnail/dbt_mods_00067076.png Comparison of Sonodynamic Treatment Set-Ups for Cancer Cells with Organic Sonosensitizers and Nanosonosensitizers. Radivoievych, Aleksandar aut Author Radivoievych Aleksandar Prylutska, Svitlana aut Author Prylutska Svitlana Zolk, Oliver aut Author 115827285 0000-0002-8220-3834 Zolk Oliver article Ritter, Uwe aut Author 137638221 0000-0002-9315-4863 Ritter Uwe Frohme, Marcus aut Author 123090210 0000-0001-9063-2682 Frohme Marcus Grebinyk, Anna aut Author 1199376795 0000-0002-4651-7908 Grebinyk Anna 2023-11-11 single unit 15 11 1 21 2616 202300000150011999 text continuingjournal Pharmaceutics journal 2009 Basel MDPI 2020-01-14 en 610 1999-4923 http://uri.gbv.de/document/gvk:ppn:614096529 2527217-2 MDPI isb Issuing body 6095067-5 126554238 en Cancer sonodynamic therapy (SDT) is the therapeutic strategy of a high-frequency ultrasound (US) combined with a special sonosensitizer that becomes cytotoxic upon US exposure. The growing number of newly discovered sonosensitizers and custom US in vitro treatment solutions push the SDT field into a need for systemic studies and reproducible in vitro experimental set-ups. In the current research, we aimed to compare two of the most used and suitable SDT in vitro set-ups - “sealed well” and “transducer in well” - in one systematic study. We assessed US pressure, intensity, and temperature distribution in wells under US irradiation. Treatment efficacy was evaluated for both set-ups towards cancer cell lines of different origins, treated with two promising sonosensitizer candidates - carbon nanoparticle C60 fullerene (C60) and herbal alkaloid berberine. C60 was found to exhibit higher sonotoxicity toward cancer cells than berberine. The higher efficacy of sonodynamic treatment with a “transducer in well” set-up than a “sealed well” set-up underlined its promising application for SDT in vitro studies. The “transducer in well” set-up is recommended for in vitro US treatment investigations based on its US-field homogeneity and pronounced cellular effects. Moreover, SDT with C60 and berberine could be exploited as a promising combinative approach for cancer treatment. Apoptosis Berberine ultrasound Sonodynamic Therapy C60 Fullerene 1875280197 2023-12-14 2025-08-01T10:42:22Z rda Converted from PICA to MODS using Pica2Mods 2.5 in mode 'DEFAULT'. http://uri.gbv.de/document/gvk:ppn:1875280197 10.3390/pharmaceutics15112616 38004594 article text 610 his Host institution dbt_mods_00067076