@Article{dbt_mods_00066812,
  author = 	{Sch{\"a}fer, Tim
		and Sherwood, Alexander
		and Kirkland, Thomas
		and Kr{\"u}ger, Thomas
		and Worbs, Jakob
		and Kniemeyer, Olaf
		and Gressler, Markus
		and Hoffmeister, Dirk},
  title = 	{In Vitro Psilocybin Synthesis by Co‐Immobilized Enzymes},
  journal = 	{Chemistry -- A European Journal},
  year = 	{2025},
  month = 	{May},
  day = 	{22},
  publisher = 	{Wiley-VCH},
  address = 	{Weinheim},
  volume = 	{31},
  number = 	{29},
  pages = 	{1--7},
  keywords = 	{biotechnology; enzyme; immobilization; natural products; psilocybin},
  abstract = 	{Advanced clinical trials investigate the Psilocybe magic mushroom natural product psilocybin as a treatment against major depressive disorder. Currently, synthetic material is used to meet the demand for legitimate pharmaceutical purposes. Here, we report an in vitro approach to biocatalytically produce psilocybin on a solid‐phase matrix charged with five covalently bound biosynthetic enzymes. These enzymes include three Psilocybe enzymes: IasA*, an engineered l ‐tryptophan decarboxylase/aromatic aldehyde synthase, the 4‐hydroxytryptamine kinase PsiK and the norbaeocystin methyltransferase PsiM, along with Escherichia coli nucleosidase MtnN and adenine deaminase Ade. In a proof‐of‐principle experiment, this enzyme‐charged resin allowed for quantitative turnover of 4‐hydroxy‐ l ‐tryptophan into psilocybin. This facile process i) represents a sustainable approach with reusable enzymes, ii) circumvents the drawbacks of in vivo processes while harnessing the selectivity of enzymatic catalysis and iii) helps access an urgently needed drug candidate.},
  note = 	{Zweitver{\"o}ffentlichung},
  issn = 	{0947-6539},
  doi = 	{10.1002/chem.202501037},
  url = 	{https://www.db-thueringen.de/receive/dbt_mods_00066812},
  url = 	{http://uri.gbv.de/document/gvk:ppn:270935193},
  url = 	{https://doi.org/10.1002/chem.202501037},
  file = 	{:https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00068459/CHEM_CHEM202501037.pdf:PDF},
  language = 	{en}
}