Direct Functionalization of Polysaccharide‐Based Xylan Phenyl Carbonate Nanoparticles with Tumor Cell Specific Antibodies

Affiliation
Surflay Nanotec GmbH 12489 Berlin Germany
Bilemjian, Vrouyr;
Affiliation
Department of Hematology University Medical Center Groningen, University of Groningen 9713 GZ Groningen The Netherlands
Lin, Yusheng;
Affiliation
Surflay Nanotec GmbH 12489 Berlin Germany
Wan, Wei;
Affiliation
Surflay Nanotec GmbH 12489 Berlin Germany
Egri, Gabriella;
Affiliation
Department of Hematology University Medical Center Groningen, University of Groningen 9713 GZ Groningen The Netherlands
Huls, Gerwin;
GND
120056801
Affiliation
Friedrich Schiller University Jena Institute of Organic Chemistry and Macromolecular Chemistry Center of Excellence for Polysaccharide Research 07743 Jena Germany
Heinze, Thomas;
Affiliation
Department of Hematology University Medical Center Groningen, University of Groningen 9713 GZ Groningen The Netherlands
Bremer, Edwin;
GND
1013355881
ORCID
0000-0002-0273-1996
Affiliation
Friedrich Schiller University Jena Institute of Organic Chemistry and Macromolecular Chemistry Center of Excellence for Polysaccharide Research 07743 Jena Germany
Gericke, Martin;
Affiliation
Surflay Nanotec GmbH 12489 Berlin Germany
Dähne, Lars

An efficient and easy‐to‐use approach is presented for obtaining biocompatible polysaccharide‐based nanoparticles (NP) that can act as tumor‐specific drug delivery agents. Two antibodies are directly immobilized onto reactive xylan phenyl carbonate (XPC) NP; namely Cetuximab (CTX) that binds to human epidermal growth factor receptor (EGFR) and Atezolizumab (ATZ) that binds to programmed death‐ligand 1 (PD‐L1). High coupling efficiency (up to 100 %) are achieved without any pre‐activation and no aggregation occurs during antibody immobilization. By quartz crystal microbalance experiments with dissipation monitoring (QCM‐D), flow cytometry assays, and confocal laser scanning microscopy imaging it is demonstrated that the functionalized XPC‐NP specifically bind to cells carrying the corresponding antigens. Moreover, the NP retain the antibody specific bioactivities (growth inhibition for CTX and induction of T‐cell cytotoxicity for ATZ).

Cite

Citation style:
Could not load citation form.

Rights

License Holder: © 2024 Wiley‐VCH GmbH

Use and reproduction: