000K  utf8
1100  2023$c2023-09-26
1500  eng
2050  urn:nbn:de:gbv:27-dbt-20231009-132029-000
2051  10.3390/ijms241914584
3000  Häring, Clio
3010  Ehrhardt, Christina
3010  Engert, Beatrice
3010  Jungwirth, Johannes
3010  Löffler, Bettina
3010  Schroeder, Josefine
4000  The Local Anaesthetic Procaine Prodrugs ProcCluster ® and Procaine Hydrochloride Impair SARS-CoV-2 Replication and Egress In Vitro  [Häring, Clio]
4060  21 Seiten
4209  As vaccination efforts against SARS-CoV-2 progress in many countries, there is still an urgent need for efficient antiviral treatment strategies for those with severer disease courses, and lately, considerable efforts have been undertaken to repurpose existing drugs as antivirals. The local anaesthetic procaine has been investigated for antiviral properties against several viruses over the past decades. Here, we present data on the inhibitory effect of the procaine prodrugs ProcCluster ® and procaine hydrochloride on SARS-CoV-2 infection in vitro. Both procaine prodrugs limit SARS-CoV-2 progeny virus titres as well as reduce interferon and cytokine responses in a proportional manner to the virus load. The addition of procaine during the early stages of the SARS-CoV-2 replication cycle in a cell culture first limits the production of subgenomic RNA transcripts, and later affects the replication of the viral genomic RNA. Interestingly, procaine additionally exerts a prominent effect on SARS-CoV-2 progeny virus release when added late during the replication cycle, when viral RNA production and protein production are already largely completed.
4950  https://doi.org/10.3390/ijms241914584$xR$3Volltext$534
4950  https://nbn-resolving.org/urn:nbn:de:gbv:27-dbt-20231009-132029-000$xR$3Volltext$534
4961  https://www.db-thueringen.de/receive/dbt_mods_00058761
5051  540
5051  570
5550  antivirals
5550  local anaesthetics
5550  procaine
5550  SARS-CoV-2