000K utf8 0100 185120377X 1100 $c2023 1500 ger 2050 urn:nbn:de:gbv:27-dbt-20230628-143701-002 3000 Wroblewski, Priscilla 4000 Charakterisierung der hippocampalen Neuro- und Gliogenese nach Sepsis in der adulten Maus [Wroblewski, Priscilla] 4060 75 Seiten 4209 Sepsis associated encephalopathy (SAE) is a major complication of patients surviving sepsis with a prevalence up to 70%. Although the initial pathophysiological events of SAE are considered to arise during the acute phase of sepsis, there is increasing evidence that SAE lead to persistent brain dysfunction with severe cognitive decline in later life. Previous studies suggest that the hippocampal formation is particularly involved leading to atrophy in later stages. Thereby, the underlying cellular mechanism are only poorly understood. Here, we hypothesized that endogenous neural stems cells and adult neurogenesis in the hippocampus are impaired following sepsis and that these changes may contribute to persistent cognitive dysfunction when the animals have physically fully recovered. We used the murine sepsis model of peritoneal contamination and infection (PCI) and combined different labeling methods of precursor cells with confocal microscopy studies to assess the neurogenic niche in the dentate gyrus at day 42 postsepsis. We found that following sepsis gliogenesis is increased, the absolute number of radial glia-like cells (type 1 cell) which are considered the putative stem cells is significantly reduced, the generation of new neurons is not significantly altered while the synpatic spine maturation of new neurons is impaired with a shift to expression of more immature and less mature spines. In conclusion, sepsis mainly leads to depletion of the neural stem cell pool and enhanced gliogenesis in the dentate gyrus which points towards an accelerated aging of the hippocampus due the septic insult. 4950 https://nbn-resolving.org/urn:nbn:de:gbv:27-dbt-20230628-143701-002$xR$3Volltext$534 4961 http://uri.gbv.de/document/gvk:ppn:185120377X 5051 610 5550 Glia 5550 Hippocampus 5550 Maus 5550 Neurogenese 5550 Sepsis