000K utf8 1100 2020$c2020-12-11 1500 eng 2050 urn:nbn:de:gbv:27-dbt-20230515-223956-005 2051 10.1038/s41598-020-78387-y 3000 von Müller, Christin 3010 Bulman, Fionnuala 3010 Eißmann, Petra 3010 Hemmerich, Peter 3010 Jacobsen, Ilse D. 3010 Kurzai, Oliver 3010 Marolda, Alessandra 3010 Perner, Birgit 3010 Rosenberger, Daniel 3010 Vylkova, Slavena 3010 Wagner, Lysett 4000 Active neutrophil responses counteract Candida albicans burn wound infection of ex vivo human skin explants [von Müller, Christin] 4060 15 Seiten 4209 Burn wounds are highly susceptible sites for colonization and infection by bacteria and fungi. Large wound surface, impaired local immunity, and broad-spectrum antibiotic therapy support growth of opportunistic fungi such as Candida albican s, which may lead to invasive candidiasis. Currently, it remains unknown whether depressed host defenses or fungal virulence drive the progression of burn wound candidiasis. Here we established an ex vivo burn wound model, where wounds were inflicted by applying preheated soldering iron to human skin explants, resulting in highly reproducible deep second-degree burn wounds. Eschar removal by debridement allowed for deeper C. albicans penetration into the burned tissue associated with prominent filamentation. Active migration of resident tissue neutrophils towards the damaged tissue and release of pro-inflammatory cytokine IL-1β accompanied the burn. The neutrophil recruitment was further increased upon supplementation of the model with fresh immune cells. Wound area and depth decreased over time, indicating healing of the damaged tissue. Importantly, prominent neutrophil presence at the infected site correlated to the limited penetration of C. albicans into the burned tissue. Altogether, we established a reproducible burn wound model of candidiasis using ex vivo human skin explants, where immune responses actively control the progression of infection and promote tissue healing. 4950 https://doi.org/10.1038/s41598-020-78387-y$xR$3Volltext$534 4950 https://nbn-resolving.org/urn:nbn:de:gbv:27-dbt-20230515-223956-005$xR$3Volltext$534 4961 https://www.db-thueringen.de/receive/dbt_mods_00057341 5051 610 5550 Experimental models of disease 5550 Fungal host response 5550 Immunology 5550 Medical research 5550 Microbiology