Development of low-cost and portable detection platforms for bacterial detection

In this study, a diagnostic platform for detecting pathogens, including Listeria monocytogenes, E. coli O157:H11, Staphylococcus aureus, Legionella spp., P. gingivalis, and Pseudomonas aeruginosa, has been developed. Fluorogenic substrates were used as a potential tool for detecting the virulence of the proteases. The identified substrates were conjugated with nanomagnetic particles to develop colorimetric, flexible, and portable platforms. The lowest detection limit of the biosensing platforms was determined as Listeria 2.17 × 102 cfu/mL,7 cfu/mL for Staphylococcus aureus and 12 cfu/mL for E. coli O157:H7, P. gingivalis was 49 cfu/mL,60 cfu/mL for Legionella spp., and 102 cfu/mL. for P. aeruginosa. The sensors were tested with other bacteria to investigate the cross-reactivity and assess the specificity; most of the sensors were found to be specific. In order to use the biosensors for field diagnosis, the sensors were evaluated using different food matrices and environmental samples from various sources. The sensors showed significant stability with all the samples. In this study, the point of interest was to devise a sensitive and quick method for detecting pathogenic bacteria in complex samples. To that end, a colorimetric sensor employing nanomagnetic particles that targeted the proteolytic activity of protease enzymes secreted by bacteria, including Listeria monocytogenes, E. coli O157:H11, Staphylococcus aureus, Legionella spp., P. gingivalis, and Pseudomonas aeruginosa, was designed. The highly specific and semi-quantitative diagnostic device developed in the current study is portable and straightforward to operate by a nurse or a non-skilled clinician, making it a potential and highly suitable system for low-resource.

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