Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli , 95.7% and 100% for Klebsiella pneumoniae , 100% and 100% for bla CTX-M , 100% and 100% for Klebsiella oxytoca , 100% and 99% for Proteus mirabilis , and 100% and 99.8% for Pseudomonas aeruginosa , respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.