Comparative Study of Different Diagnostic Routine Methods for the Identification of Acinetobacter radioresistens

Affiliation
Institute for Microbiology and Virology, University Hospital Carl Gustav Carus, 01307 Dresden, Germany
Bigge, Richard;
ORCID
0000-0002-8420-8161
Affiliation
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany
Bunk, Boyke;
ORCID
0000-0002-6300-4717
Affiliation
Department of Hospital Infection Control, University Hospital Carl Gustav Carus, 01307 Dresden, Germany
Rudolph, Wolfram W.;
Affiliation
Department of Hospital Infection Control, University Hospital Carl Gustav Carus, 01307 Dresden, Germany
Gunzer, Florian;
GND
130665053
ORCID
0000-0002-7130-0006
Affiliation
Jena University Hospital
Coldewey, Sina M.;
Affiliation
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany
Riedel, Thomas;
Affiliation
Institute for Microbiology and Virology, University Hospital Carl Gustav Carus, 01307 Dresden, Germany
Schröttner, Percy

Recent publications indicate that A. radioresistens can cause infections in humans, even though it is rarely reported in routine diagnostics. However, the fact that it is infrequently detected may be explained by the misidentification of the species by conventional methods. It is also likely that A. radioresistens is not considered clinically relevant and therefore not consistently included in diagnostic results. To elucidate the medical significance of this probably clinically underestimated bacterial species, we created a well-documented reference strain collection of 21 strains collected in routine diagnostics. For further analysis of A. radioresistens , it is essential to know which methods can be used to achieve a trustworthy identification. We, therefore, compared three methods widely used in routine diagnostics (MALDI-TOF MS, VITEK 2, and sequencing of housekeeping genes) in terms of secure and reliable identification of A. radioresistens . As reference methods, whole genome-based approaches were applied. VITEK 2 led to misidentification for four strains. However, MALDI-TOF MS and sequencing of housekeeping genes led to reliable and robust identifications.

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