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MatriGrid® based biological morphologies : tools for 3D cell culturing

Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Mai, Patrick;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Hampl, Jörg;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Baca, Martin;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Brauer, Dana;
ORCID
0000-0001-6871-4294
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Singh, Sukhdeep;
ORCID
0000-0002-4513-987X
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Weise, Frank;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Borowiec, Justyna;
Affiliation
Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747 Jena, Germany;(A.S.);(U.R.M.)
Schmidt, André;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Küstner, Johanna Merle;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Klett, Maren;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Gebinoga, Michael;
Affiliation
Biophysics Division, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, Germany;
Schroeder, Insa S.;
ORCID
0000-0001-5299-595X
Affiliation
Placenta Lab, Department of Obstetrics, Jena University Hospital, 07747 Jena, Germany;(A.S.);(U.R.M.)
Markert, Udo R.;
Affiliation
Institute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany;(F.G.);(B.S.);(D.E.)
Glahn, Felix;
Affiliation
Institute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany;(F.G.);(B.S.);(D.E.)
Schumann, Berit;
Affiliation
Institute of Environmental Toxicology, Martin-Luther-University Halle-Wittenberg, 06097 Halle, Germany;(F.G.);(B.S.);(D.E.)
Eckstein, Diana;
Affiliation
Department of Nano-Biosystems Engineering, Institute of Chemistry and Biotechnology, Ilmenau University of Technology, 98693 Ilmenau, Germany;(P.M.);(M.B.);(D.B.);(S.S.);(F.W.);(J.B.);(J.M.K.);(M.K.);(M.G.)
Schober, Andreas

Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrid ® s. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account.

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Published in:
Bioengineering : open access journal
Vol. 9, No. 5 (20.05.2022)Art.-No.:220
Volume:
9
No.:
5
Date Created:
06.07.2022
Date Issued:
20.05.2022
DOI:
10.3390/bioengineering9050220
PPN:
180247837X
Language:
English
Type of Resource:
Text
Extent:
41 Seiten
Keywords:
  1. scaffolds for 3D cell culture
    Typ
    Schlagwort
  1. hepatocyte culture
    Typ
    Schlagwort
  1. scaffold manufacturing
    Typ
    Schlagwort
  1. manipulation of organoids
    Typ
    Schlagwort
  1. stem cell niches
    Typ
    Schlagwort
  1. neurons and cerebral bodies
    Typ
    Schlagwort
  1. automated cell culturing unit
    Typ
    Schlagwort
  1. 3D micropattern technique
    Typ
    Schlagwort
  1. microcontact printing
    Typ
    Schlagwort
  1. thermoforming
    Typ
    Schlagwort
  1. cell adhesion
    Typ
    Schlagwort
  1. extracellular matrix
    Typ
    Schlagwort
DDC subject of DNB:
570 Life science
Hosting institution:
Technische Universität Ilmenau, Fakultät für Mathematik und Naturwissenschaften

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