@Article{dbt_mods_00051275,
  author = 	{Wiegand, Cornelia
		and Hipler, Uta-Christina
		and Elsner, Peter
		and Tittelbach, J{\"o}rg},
  editor = 	{Venihaki, Maria},
  title = 	{Keratinocyte and Fibroblast Wound Healing In Vitro Is Repressed by Non-Optimal Conditions but the Reparative Potential Can Be Improved by Water-Filtered Infrared A},
  journal = 	{Biomedicines: open access journal},
  year = 	{2021},
  month = 	{Nov},
  day = 	{30},
  publisher = 	{MDPI},
  address = 	{Basel},
  volume = 	{9},
  number = 	{12},
  pages = 	{1--34},
  keywords = 	{wound healing; scratch assay; keratinocyte; fibroblast; UV-B; wIRA (water-filtered infrared A); pro-inflammatory cytokines; heat shock protein; desmoglein; collagen},
  abstract = 	{It is a general goal to improve wound healing, especially of chronic wounds. As light therapy has gained increasing attention, the positive influence on healing progression of water-filtered infrared A (wIRA), a special form of thermal radiation, has been investigated and compared to the detrimental effects of UV-B irradiation on wound closure in vitro. Models of keratinocyte and fibroblast scratches help to elucidate effects on epithelial and dermal healing. This study further used the simulation of non-optimal settings such as S. aureus infection, chronic inflammation, and anti-inflammatory conditions to determine how these affect scratch wound progression and whether wIRA treatment can improve healing. Gene expression analysis for cytokines ( IL1A , IL6 , CXCL8 ), growth ( TGFB1 , PDGFC ) and transcription factors ( NFKB1 , TP53 ), heat shock proteins ( HSP90AA1 , HSPA1A , HSPD1 ), keratinocyte desmogleins ( DSG1 , DSG3 ), and fibroblast collagen ( COL1A1 , COL3A1 ) was performed. Keratinocyte and fibroblast wound healing under non-optimal conditions was found to be distinctly reduced in vitro. wIRA treatment could counteract the inflammatory response in infected keratinocytes as well as under chronic inflammatory conditions by decreasing pro-inflammatory cytokine gene expression and improve wound healing. In contrast, in the anti-inflammatory setting, wIRA radiation could re-initiate the acute inflammatory response necessary after injury to stimulate the regenerative processes and advance scratch closure.},
  issn = 	{2227-9059},
  doi = 	{10.3390/biomedicines9121802},
  url = 	{https://www.db-thueringen.de/receive/dbt_mods_00051275},
  url = 	{http://uri.gbv.de/document/gvk:ppn:1786285649},
  url = 	{http://uri.gbv.de/document/gvk:ppn:750370483},
  url = 	{https://doi.org/10.3390/biomedicines9121802},
  file = 	{:https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00055553/biomedicines-09-01802.pdf:PDF},
  language = 	{en}
}