https://www.db-thueringen.de/receive/dbt_mods_00049514https://www.db-thueringen.de/servlets/MCRFileNodeServlet/dbt_derivate_00054017/TPJ_TPJ15161.pdfhttps://www.db-thueringen.de/rsc/thumbnail/dbt_mods_00049514.png text article Aethionema arabicum genome annotation using pacbio full‐length transcripts provides a valuable resource for seed dormancy and brassicaceae evolution research Fernandez‐Pozo, Noe Fernandez‐Pozo Noe University of Marburg aut Author 0000-0002-6489-5566 Metz, Timo Metz Timo University of Marburg aut Author 0000-0003-4213-4907 Chandler, Jake O. Chandler Jake O. Royal Holloway University of London aut Author 0000-0003-0955-9241 article Gramzow, Lydia Gramzow Lydia Matthias Schleiden Institute/Genetics aut Author 0000-0003-1919-6077 1014065593 Mérai, Zsuzsanna Mérai Zsuzsanna Austrian Academy of Sciences aut Author 0000-0002-2048-1628 Maumus, Florian Maumus Florian Université Paris‐Saclay aut Author 0000-0001-7325-0527 Mittelsten Scheid, Ortrun Mittelsten Scheid Ortrun Austrian Academy of Sciences aut Author 0000-0002-7757-4809 Theißen, Günter Theißen Günter Matthias Schleiden Institute/Genetics aut Author 0000-0003-4854-8692 1027426107 Schranz, M. Eric Schranz M. Eric Wageningen University aut Author 0000-0001-6777-6565 urn:nbn:de:gbv:27-dbt-20210907-163949-002 Leubner‐Metzger, Gerhard Leubner‐Metzger Gerhard Royal Holloway University of London Palacký University and Institute of Experimental Botany aut Author 0000-0002-6045-8713 Rensing, Stefan A. Rensing Stefan A. University of Marburg University of Freiburg University of Marburg aut Author 0000-0002-0225-873X 10.1111/tpj.15161 2021-04-16 Oxford Wiley-Blackwell 106, 2021 1 275 293 continuingjournal journal The plant journal Plant J 0960-7412 1365-313X text 1991 Oxford Wiley-Blackwell en 580 Aethionema arabicum is an important model plant for Brassicaceae trait evolution, particularly of seed (development, regulation, germination, dormancy) and fruit (development, dehiscence mechanisms) characters. Its genome assembly was recently improved but the gene annotation was not updated. Here, we improved the Ae. arabicum gene annotation using 294 RNA‐seq libraries and 136 307 full‐length PacBio Iso‐seq transcripts, increasing BUSCO completeness by 11.6% and featuring 5606 additional genes. Analysis of orthologs showed a lower number of genes in Ae. arabicum than in other Brassicaceae, which could be partially explained by loss of homeologs derived from the At‐α polyploidization event and by a lower occurrence of tandem duplications after divergence of Aethionema from the other Brassicaceae. Benchmarking of MADS‐box genes identified orthologs of FUL and AGL79 not found in previous versions. Analysis of full‐length transcripts related to ABA‐mediated seed dormancy discovered a conserved isoform of PIF6‐β and antisense transcripts in ABI3 , ABI4 and DOG1 , among other cases found of different alternative splicing between Turkey and Cyprus ecotypes. The presented data allow alternative splicing mining and proposition of numerous hypotheses to research evolution and functional genomics. Annotation data and sequences are available at the Ae . arabicum DB ( https://plantcode.online.uni‐marburg.de/aetar_db ). Significance Statement Improved gene annotation of Aethionema arabicum using long‐read transcript sequencing provides a plethora of full‐length isoforms and an important resource for Brassicaceae evolution studies. Aethionema arabicum genome annotation seed germination Brassicaceae evolution alternative splicing transcription factors Iso‐seq en 19 Seiten 580 his Host institution Zweitveröffentlichung dbt_mods_00049514https://www.db-thueringen.de/receive/dbt_mods_00049514