Phosphorylation-dependent differences in CXCR4-LASP1-AKT1 Interaction between breast cancer and chronic myeloid leukemia

Butt, Elke GND; Stempfle, Katrin; Lister, Lorenz; Wolf, Felix; Kraft, Marcella; Herrmann, Andreas B.; Viciano, Cristina Perpina; Weber, Christian; Hochhaus, Andreas GND; Ernst, Thomas GND; Hoffmann, Carsten; Zernecke, Alma GND; Frietsch, Jochen J. GND

The serine/threonine protein kinase AKT1 is a downstream target of the chemokine receptor4 (CXCR4), and both proteins play a central role in the modulation of diverse cellular processes,including proliferation and cell survival. While in chronic myeloid leukemia (CML) the CXCR4is downregulated, thereby promoting the mobilization of progenitor cells into blood, the receptoris highly expressed in breast cancer cells, favoring the migratory capacity of these cells. Recently,the LIM and SH3 domain protein 1 (LASP1) has been described as a novel CXCR4 binding partnerand as a promoter of the PI3K/AKT pathway. In this study, we uncovered a direct binding ofLASP1, phosphorylated at S146, to both CXCR4 and AKT1, as shown by immunoprecipitation assays,pull-down experiments, and immunohistochemistry data. In contrast, phosphorylation of LASP1at Y171 abrogated these interactions, suggesting that both LASP1 phospho-forms interact. Finally,findings demonstrating different phosphorylation patterns of LASP1 in breast cancer and chronicmyeloid leukemia may have implications for CXCR4 function and tyrosine kinase inhibitor treatment.

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