Molekulare Analyse der sexuellen Kommunikation des Zygomyzeten Mucor mucedo
At the genomic level several differences between the Mucor mucedo mating types (-) and (+) are visible (Abb. 4.54). At the protein level, however, only few differences are measurable (Abb. 4.1 – 4.3). Protein sequences found by MALDI-TOF analysis are an indication that especially in the (-)- mating type many membrane – associated proteins are activated. The activation of the citric acid cycle and the fatty acid ß-oxidation in the (+)- mating type are evidence to suggest that there is an elevated energy demand. DNA sequences results of the cloned fragments of Mucor mucedo show in comparison to gene sequences of basidiomycetes, ascomycetes and other zygomycetes (Abb. 4.48-4.53) that Mucor mucedo differs considerably from other fungi. A sequence comparison with the zygomycetes Mucor circinelloides, Phycomyces blakesleeanus and Rhizopus oryzae shows that they have significant differences which make it conceivable that there are considerable differences in the amino acid sequences at the protein level as well. This shows that it is difficult to receive reliable results in the MALDI-TOF analysis. Nevertheless this only seems to concern the cell wall associated proteins which very often have posttranslational modifications. These impede the MALDI-TOF method. Within the cytoplasmatic proteins four sequences were unambiguously identifiable since these amino acid sequences are highly conserved. The chitosan staining results indicate that chitosan is necessary for the fusion of both mating partners. It has to be evaluated how many and which chitin deacetylases are present in Mucor mucedo. Subsequently double- or multiple knock-outs should be constructed to describe the phenotype. It is possible that the respective mating partners will then not be able to form zygospores.