Origins and consequences of persistent stat3 activation in colorectal carcinoma cells
A very interesting emerging target in cancer therapy is the signal transducer and activator of transcription - 3 (Stat3). One major purpose of this work is an analysis of functions of Stat3-activation in colorectal cancer cells, by studying its influence on parameters such as cell proliferation, invasion properties, and anchorage-independent growth. Moreover, the project intends to correlate the expression of malignancy-associated proteinases with aberrant Stat3 activity, thereby possibly identifying novel target genes of Stat3 in the context of CRC. Another goal of this work is to investigate potential origins of persistent Stat3 activation in colorectal cancer tissue. Methods of discriminating between paracrine and autocrine mechanisms of Stat3 are to be developed with the goal of obtaining information on the nature of the factor(s) that induce Stat3 activity in CRC cells.